发布日期：2025-01-04 15:59 点击次数：88
1. 保存条件：建议 -20 °C保存。2. mycin，霉素，在不严格的缩写中作为“XX霉素”的后缀缩写为m。3. 氯霉素（Chlor Am Phenicol），氯氨苯醇，标准缩写CAP，有时也写做Cm。4. 过滤灭菌：用0.22 μm一次性滤膜过滤除菌，于超净台内。5. 母液配制方法举例，如100 mg/mL氨苄青霉素：溶解1 g氨苄青霉素钠盐于足量的水中，最后定容至10 mL。分装成小份于-20 °C贮存。常以25 μg/mL～50 μg/mL的终浓度添加于生长培养基1. 50× TAE（1 L）：2. 10× TBE（1 L）：3. 6×DNA Loading Buffer（100 mL）：4. 0.5 M EDTA pH 8.0（500 mL）：5. X-Gal （40 mg/mL）, 1000×（5 mL）6. SOC medium（250 mL）7. Solutions for Plasmid Preparations1) Qiagen Buffer P1：50 mM Tris-HCl pH 8.0，10 mM EDTA，100 μg/mL RNaseAadd ddH2O to 100 mL, Autoclave. Rnase A (add after autoclaving) 10.0 mg. Store at 4 °C2) Qiagen Buffer P2：200 mM NaOH，1% SDS3) Qiagen Buffer P3：3.0 M potassium acetate pH 5.5,4) Qiagen Buffer N3：4.2 M Gu-HCl，0.9 M potassium acetate pH 4.8 5) Qiagen Buffer PB（5 M Gu-HCl, 30% isopropanol）＝PBI（I for pH indicator）6) Qiagen Buffer QG（5.5 M guanidine thiocyanate （GuSCN），20 mM Tris HCl pH 6.6）7) Qiagen Buffer PE（10 mM Tris-HCl pH 7.5，80% ethanol ）8) Qiagen Buffer QX1：7 M NaPO4，10 mM NaAc pH 5.3 9) Qiagen Buffer QXB：5 M GuHCl 10) Qiagen Buffer QBT：750 mM NaCl，50 mM MOPS pH 7.0，15% isopropanol，0.15% triton X-10011) Qiagen Buffer QC：1.0 M NaCl，50 mM MOPS pH 7.0，15% isopropanol12) Qiagen Buffer QF：1.25 M NaCl， 50 mM Tris-HCl pH 8.5，15% isopropanol8. Buffers for Protein Electrophoresis1) 6×SDS Sample Buffer2) Coommassie Blue Staining Solution（500 mL）：3) Coommassie Blue Destaining Solution （500 mL）：4) 20×MES-SDS running buffer （1 L）：MES：2-(N-吗啉)乙磺酸一水合物9. Molecular Biology Buffers:1) A1：1×NEBuffer 1（yellow）：10 mM Bis Tris Propane-HCl，10 mM MgCl2，1 mM DTT pH 7.0 @ 25 °C。Supplied as a 10×concentrated stock.2) A2：1×NEBuffer 2（blue）：50 mM NaCl, 10 mM Tris-HCl，10 mM MgCl2，1 mM DTT pH 7.9 @ 25 °C。Supplied as a 10×concentrated stock.3) A2：1×NEBuffer 3（red）：100 mM NaCl, 50 mM Tris-HCl，10 mM MgCl2，1 mM DTT pH 7.9 @ 25 °C。Supplied as a 10×concentrated stock.4) A2：1×NEBuffer 4（green，cutsmart buffer）：50 mM potassium acetate，20 mM Tris acetate，10 mM magnesium acetate，1 mM DTT, pH 7.9 @ 25 °C。Supplied as a 10×concentrated stock.5) 2×T4 DNA quick ligase 快连接酶buffer：60 mM Tris-HCl pH 7.8，20 mM MgCl2，2 mM DTT，2 mM ATP and 10% PEG6000 6) 10×T4 DNA连接酶buffer：500 mM Tris-HCl pH 7.6，100 mM MgCl2，100 mM DTT，10 mM ATP7) 10×pfu buffer：200 mM Tris-HCl pH 8.6，100 mM KCl，160 mM (NH4)2SO4，10 mM MgCl2，1% Triton X-100，1 mg/mL BSA。8) 5×HF buffer：32 mM HEPES-KOH buffer pH 7.8，100 mM KAcO, 4 mM Mg (AcO) 2，0.05% BSA（0.5 mg/mL）9) 5×GC rich buffer：32 mM HEPES-KOH buffer pH 7.8，100 mM KAcO，7.5 mM Mg(AcO)2，0.01% BSA（0.1 mg/mL），1.0% DMSO10) 2×Taq mix:11) 10×Taq buffer:材料（MATERIALS）r 试剂（REAGENTS）NaH2PO4·2H2O、Na2HPO4·12H2O配制方法：1. 0.2 M的 NaH2PO4：称取NaH2PO4.2H2O 31.21 g（或 NaH2PO4·H2O 27.6 g）加重蒸水至1000 mL溶解。2. 0.2 M的 Na2HPO4：称取Na2HPO4·12H2O 71.64 g（或 Na2HPO4·7H2O 53.6 g或 Na2HPO4·2H2O 35.61 g）加重蒸水至1000 mL溶解。3. 0.2 M pH 7.4的PB的配制：取19 mL 0.2 M的NaH2PO4和81 mL 0.2 M Na2HPO4，充分混合即为0.2 M的PB（pH约为7.4～7.5）。若pH偏高或偏低，可通过改变二者的比例来加以调整，室温保存即可。► 25 °C下0.2 M磷酸钠缓冲液的配制► 25 °C下0.1 M磷酸钾缓冲液的配制1. 分子克隆（Molecular Cloning）1) 引物Tm值计算：2) 密码子优化：https://sg.idtdna.com/CodonOpt3) 质粒图谱分析：4) 启动子信息：https://blog.addgene.org/plasmids-101-the-promoter-region5) addgene（一个质粒数据库）：-database/6) NEB 内切酶buffer推荐：https://nebcloner.neb.com/#!/redigest7) 核酸数据分析：2. 蛋白表达与纯化（Protein Expression and Purification）1) 蛋白表达菌株信息：-strains-prot-exp.html2) 蛋白纯化：_Protocols.html3. 蛋白序列分析（Protein Sequence Analysis）1) 信号肽预测：2) 跨膜区预：TMpred _form.html3) 结构域预测：-heidelberg.de/4) 蛋白性质预测： 5) 蛋白质结构与功能预测：6) 二级结构预测：7) 评估结构预测的准确性和可靠性：https://www.cameo3d.org/8) 蛋白质结构和功能预测：https://open.predictprotein.org/9) 三维结构比对_server/start10) 同源建模https://zhanglab.ccmb.med.umich.edu/I-TASSER/11) 同源建模：https://swissmodel.expasy.org/12) 预测蛋白相互作用：https://string-db.org/13) Pfam数据库是蛋白质家族的集合（根据结构域分类）：4. 结构分析（Structure Analysis)1) RCSB PDB 数据库：2) 晶体界面分析 PISA server3) Deposition serverhttps://deposit-1.wwpdb.org/4) 配体结构文件下载：5) Bernhard Rupp的生物大分子晶体学网站：5. 其他1) ATCC：2) 化学分子性质：_EN.aspx3) RNA修饰数据库：4) Wikipedia：https://www.wikipedia.org/5) 各类protocols：6) 文献查询：https://sci-hub.tw/1. 由于衍射仪测角头电机比较小，如果手动上样的话很容易把位置碰歪，所以目前只能机械手上样，建议各位老师将晶体样品保存在puck里，如果没有puck，可以提前到线站借用我们线站的puck将晶体样品转移到puck里再通过机械手上样。2. 如果在实验的过程中发生故障，一定要第一时间给值班人员打电话，不能自行去处理。3. 建议在EIGER 16 M探测器上收数据的策略是：0.1~0.3度一张，曝光时间0.05秒一张。这样收一套360度的完整数据（3600张衍射图）大概只需要3分钟。4. 一套3600张衍射图的数据大小是25 GB左右，建议带一个存储空间大的硬盘拷贝数据，可以边实验边拷贝数据。减少拷贝数据等待时间。5. 数据处理建议使用XDS。6. 目前线站的新系统还需要进一步优化调试，能量暂时固定在12.660 Kev。NameBacknoneDescriptionCloning FrameFusion TagSequencing Primer 1Sequencing Primer2Resistance V1pET30aNdeI-SalI,NotI-XhoI-His6-stopSalI-NotInoneT7 PromoterT7 TerminatorKan V2pET30aNdeI-His8-SalI,NotI-stopSalI-NotInoneT7 PromoterT7 TerminatorKan V3pET30aNdeI-His8-GB1-TEV-SalI,NotI-stopSalI-NotIHis8-GB1-TEVT7 PromoterT7 TerminatorKan V4pET30aNdeI-His8-GB1-TEV-SalI,NotI-XhoI-His6-stopSalI-NotIHis8-GB1-TEVT7 PromoterT7 TerminatorKan V5pET30aNdeI-His8-TEV-SalI,NotI-stopSalI-NotIMGSS-His8-TEVT7 PromoterT7 TerminatorKan V6pET30aNdeI-His8-TEV-SalI,NotI-XhoI-His6-stopSalI-NotIMGSS-His8-TEVT7 PromoterT7 TerminatorKan V7pET30aNdeI-His8-SUMO-TEV-SalI,NotI-XhoI-stopSalI-NotIMGSS-His8-SUMO-TEVT7 PromoterT7 TerminatorKan V8pET30aNdeI-His8-VHb-TEV-SalI,NotI-stopSalI-NotIHis8-VHb-TEVT7 PromoterT7 TerminatorKan V9pET30aNdeI-His8-VHb-TEV-SalI,NotI-XhoI-His6-stopSalI-NotIHis8-VHb-TEVT7 PromoterT7 TerminatorKan V10pET30aNdeI-His8-GST-TEV-SalI,NotI-stopSalI-NotIHis8-GST-TEVT7 PromoterT7 TerminatorKan V11pET30aNdeI-His8-GST-TEV-SalI,NotI-XhoI-His6-stopSalI-NotIHis8-GST-TEVT7 PromoterT7 TerminatorKan V12pET30aNdeI-His7-TRX-TEV-SalI,NotI-stopSalI-NotIHis7-TRX-TEVT7 PromoterT7 TerminatorKan V13pET30aNdeI-His7-TRX-TEV-SalI,NotI-XhoI-His6-stopSalI-NotIHis7-TRX-TEVT7 PromoterT7 TerminatorKan V14pET30aNdeI-His8-MBP-SalI,NotI-stopSalI-NotIHis8-MBPT7 PromoterT7 TerminatorKan V15pET30aNdeI-His8-tag2-TEV-SalI,NotI-stopSalI-NotIHis8-tag2-TEVT7 PromoterT7 TerminatorKan V16pET30aNdeI-His8-tag2-TEV-SalI,NotI-XhoI-His6-stopSalI-NotIHis8-tag2-TEVT7 PromoterT7 TerminatorKan V17pET30aNdeI-DsbA-TEV-SalI,NotI-XhoI-His6-stopSalI-NotIDsbA-TEVT7 PromoterT7 TerminatorKan V18pET30aNdeI-MBP-SalI,NotI-XhoI-His6-stopSalI-NotIMBPT7 PromoterT7 TerminatorKan V19pET30aNdeI-His8-NusA-TEV-SalI,NotI-stopSalI-NotIHis8-NusA-TEVT7 PromoterT7 TerminatorKan V20pET30aNdeI-His8-GB1-TEV-SalI,NotI-Acidic Peptide-LacZ-XhoI-His6-stopSalI-NotIHis8-GB1-TEVT7 PromoterT7 TerminatorKan V21pET30aNdeI-His8-MBP-helical linker-SalI,NotI-stopSalI-NotIHis8-MBP-helical linkerT7 PromoterT7 TerminatorKan V22pMALNdeI-MBP-SalI,NotI-XhoI-LacZ-stopSalI-NotIMBPM13R-pUCM13F-pUCAmp V23pMALNdeI-MBP-SalI,NotI-XhoI-LacZ-stopSalI-NotIMBPM13R-pUCM13F-pUCAmp V24pMALNdeI-MBP-SalI,NotI-XhoI-LacZ-stopSalI-NotIMBPM13R-pUCM13F-pUCAmp V25pMALNdeI-MBP-SalI,NotI-XhoI-LacZ-stopSalI-NotIMBPM13R-pUCM13F-pUCAmp V26pMALNdeI-MBP-SalI,NotI-XhoI-LacZ-stopSalI-NotIMBPM13R-pUCM13F-pUCAmp V27pMALNdeI-MBP-SalI,NotI-XhoI-LacZ-stopSalI-NotIMBPM13R-pUCM13F-pUCAmp V28pET30aNdeI-MBP-SalI,NotI-XhoI-His6-stopSalI-NotIMBPT7 PromoterT7 TerminatorKan V29pET30aNdeI-MBP-TEV-SalI,NotI-stopSalI-NotIMBP-TEVT7 PromoterT7 TerminatorKan V29HpET30aNdeI-His8-MBP-TEV-SalI,NotI-stopSalI-NotIHis8-MBP-TEVT7 PromoterT7 TerminatorKan V30pET30aNdeI-MBP-TEV-SalI,NotI-XhoI-His6-stopSalI-NotIMBP-TEVT7 PromoterT7 TerminatorKan V31pLEXSY-BleNcoI-SP-SalI,NotI-His6-stopSalI-NotISignal PeptideP14A26Amp/Ble/Tet V32pLEXSY-BleNcoI-SP-Strep Tag-His8-TEV-SalI,NotI-His6-stopSalI-NotISP-Strep Tag-His8-TEVP14A26Amp/Ble/Tet V33pLEXSY-BleNcoI-SalI,NotI-His8-stopSalI-NotInoneP14A26Amp/Ble/Tet V34pLEXSY-BleNcoI-Strep Tag-SalI,NotI-His8-stopSalI-NotIStrep TagP14A26Amp/Ble/Tet V35pLEXSY-BleNcoI-His8-GST-TEV-SalI,NotI-stopSalI-NotIHis8-GST-TEVP14A26Amp/Ble/Tet V36pLEXSY-BleNcoI-SP-Strep-His8-TEV-SalI,NotI-His6-stopSalI-NotISP-Strep-His8-TEVP14A26Amp/Ble/Tet V37pLEXSY-BleNcoI-Strep-His8-TEV-SalI,NotI-His6-stopSalI-NotIStrep-His8-TEVP14A26Amp/Ble/Tet V40pTT5NcoI-His8-GB1-TEV-SalI,NotI-stopSalI-NotIHis8-GB1-TEVT7 PromoterT7 TerminatorKan V41pTT5NcoI-His6-Strep-His8-TEV-SalI,NotI-stopSalI-NotIHis6-Strep-His8-TEVP14A26Amp/Ble/Tet V42pTT5NcoI-Strep-His8-TEV-SalI,NotI-stopSalI-NotIStrep-His8-TEVP14A26Amp/Ble/Tet V51pFabEcoRV-SP-Strep-His8-TEV-SalI,NotI-His6-stopSalI-NotISP-Strep-His8-TEVunknownunknownAmp V52pFabEcoRV-SP-His6-TEV-SalI,NotI-stopSalI-NotISP-His6-TEVunknownunknownAmp V53pFabEcoRV-SP-His6-TEV-SalI,NotI-stopSalI-NotISP-His6-TEVunknownunknownAmp V54pFabEcoRV-SP-His6-TEV-SalI,NotI-Strep-stopSalI-NotISP-His6-TEVunknownunknownAmp V55pMALNdeI-MBP-TEV-SalI,NotI-stopSalI-NotIMBP-TEVM13R-pUCM13F-pUCAmp V56pMW103LexA-SalI,NotI-stopSalI-NotILexAunknownunknownKanFor Y2H V57pET30aNdeI-hDim2-SalI,NotI-XhoI-His6-stopSalI-NotIhDim2T7 PromoterT7 TerminatorKan V59pET30aNdeI-His8-TEV-SalI,NotI-Lysozyme-stopSalI-NotIHis8-TEVT7 PromoterT7 TerminatorKan V60pET30aNdeI-Lysozyme-SalI,NotI-XhoI-His6-stopSalI-NotILysozymeT7 PromoterT7 TerminatorKan V61pEU-01His6-TEV-SalI,NotI-stopSalI-NotIHis6-TEVunknownunknownAmp V62pET30aNdeI-MBP-SalI,NotI-lysozyme-XhoI-His6-stopSalI-NotIMBPT7 PromoterT7 TerminatorKan V63pET30aNdeI-MBP-TEV-SalI,NotI-Lysozyme-XhoI-His6-stopSalI-NotIMBP-TEVT7 PromoterT7 TerminatorKan V64pET30aNdeI-His8-MBP-TEV-SalI,NotI-Lysozyme-stopSalI-NotIHis8-MBP-TEVT7 PromoterT7 TerminatorKan V65pJG4-5Gal1-B42-HA epitope-EcoRI-SalI,NotI-stopSalI-NotIGal1-B42-HA epitopeunknownunknownAmpFor Y2H V66pET30aNdeI-T4LS5-SalI,NotI-XhoI-His6-stopSalI-NotIT4LS5T7 PromoterT7 TerminatorKan V67pEU-01NdeI-His8-NcoI-TEV-SalI,NotI-stopSalI-NotIHis8-TEVT7 PromoterT7 TerminatorKan V71pET30aNdeI-SalI,NotI-XhoI-His6-stopSalI-NotInoneT7 PromoterT7 TerminatorKan V72pET30aNdeI-SalI,NotI-Strep-stopSalI-NotInoneT7 PromoterT7 TerminatorKan V77pBADNdeI-SalI,NotI-XhoI-His6-stopSalI-NotInonepBAD forpBAD revAmp V78pBAD-V28ENdeI-MBP-SalI,NotI-XhoI-His6-stopSalI-NotIMBPpBAD forpBAD revAmp V79pBAD-V29HNdeI-His8-MBP-TEV-SalI,NotI-stopSalI-NotIHis8-MBP-TEVpBAD forpBAD revAmp V80pCMV-HAHAtag-EcoRI-SalI,NotI-stopSalI-NotIHAtagpCMVM13R-20Amp V81pBAD-V30NdeI-MBP-TEV-SalI,NotI-XhoI-His6-stopSalI-NotIMBP-TEVpBAD forpBAD revAmp V82pBAD-V29HSNdeI-His8-MBP-TEV-SalI,NotI-strep-stopSalI-NotIHis-MBP-TEVpBAD forpBAD revAmp V83pASK-17plusNdeI-Strep-TEV-EcoRI-BamHI-XhoI-SalI-NcoI-EcoRV-HindIIIMCSStrep-TEVpASk forpASK revAmp V84pASK-V3NdeI-His8-GB1-TEV-SalI,NotI-stopSalI-NotIHis8-GB1-TEVpASk forpASK revAmp V85pASK-V28ENdeI-MBP-SalI,NotI-XhoI-His6-stopSalI-NotIMBPpASk forpASK revAmp V86pASK-V29HNdeI-His8-MBP-TEV-SalI,NotI-stopSalI-NotIHis-MBP-TEVpASk forpASK revAmp V87pASK-V30NdeI-MBP-TEV-SalI,NotI-XhoI-His6-stopSalI-NotIMBP-TEVpASk forpASK revAmp V88pASK-V29HSNdeI-His8-MBP-TEV-SalI,NotI-Strep-stopSalI-NotIHis-MBP-TEVpASk forpASK revAmp V89pET30aNdeI-InfB21-eGFP-SalI,NotI-stopSalI-NotIInfB21-eGFPT7 PromoterT7 TerminatorKan V90pET30aNdeI-InfB21-eGFP-TEV-SalI,NotI-stopSalI-NotIInfB21-eGFPT7 PromoterT7 TerminatorKan V91pET30aNdeI-His8-eGFP-TEV-SalI,NotI-stopSalI-NotIHis8-eGFP-TEVT7 PromoterT7 TerminatorKan V92pET30aNdeI-SUMO-SalI,NotI-Strep-stopSalI-NotISUMOT7 PromoterT7 TerminatorKan V93pET30aNdeI-His8-MBP-TEV-GFP-SalI,NotI-Strep-stopSalI-NotIHis8-MBP-TEV-GFPT7 PromoterT7 TerminatorKan V94pET30aNdeI-VHb-SalI,NotI-Strep-stopSalI-NotIVHbT7 PromoterT7 TerminatorKan V95pET30aNdeI-Tag2-SalI,NotI-Strep-stopSalI-NotITag2T7 PromoterT7 TerminatorKan V96pET30aNdeI-MBP-SalI,NotI-XhoI-His6-stopSalI-NotIMBPT7 PromoterT7 TerminatorKan V97pET30aNdeI-MBP-SalI,NotI-Strep-stopSalI-NotIMBPT7 PromoterT7 TerminatorKan V98pFabEcoRV-SP-NdeI-His8-SalI,NotI-Strep-stopSalI-NotISP-His8unknownunknownAmp V99pET30aNdeI-MBP-SalI,NotI-XhoI-His6-stopSalI-NotIMBPT7 PromoterT7 TerminatorKan V100pET30aNdeI-MBP-SalI,NotI-XhoI-His6-stopSalI-NotIMBPT7 PromoterT7 TerminatorKan V101pET30aNdeI-GlyA-SalI,NotI-XhoI-His6-stopSalI-NotIGlyAT7 PromoterT7 TerminatorKan V102pET30aNdeI-GacH-SalI,NotI-XhoI-His6-stopSalI-NotIGacHT7 PromoterT7 TerminatorKan V103pET30aNdeI-XBP1-SalI,NotI-XhoI-His6-stopSalI-NotIXBP1T7 PromoterT7 TerminatorKan V104pGBKT7SNDNA Binding-XhoI-NdeI-SalI,NotI-stopSalI-NotIDNA BindingT7 PromoterADHKan V105pGADT7SNSV40-AD-NcoI-Epitope-NdeI-SalI,NotI-stopSalI-NotISV40-AD-EpitopeT7 PromoterADHAmp V106pRmHa3EcoRI-TEV-SalI,NotI-XhoI-His6-stopSalI-NotITEVpRmHa3 forpRmHa3 revAmp V107pVL1392NcoI-NotI-HIs6-stopunknownnoney326 polyhedriny327 polyhedrinAmp V108plSUMOstarHIs6-SUMOstar-SalI,NotI-stopSalI-NotISUMOstarunknownunknownAmp/Get V109pFastBacHTNdeI-InfB21-His8-TEV-SalI,NotI-stopSalI-NotIInfB21-His8-TEVpASk forpASK revAmp/Get V110pFastBacHTNdeI-SalI,NotI-XhoI-His6-stopSalI-NotInonepASk forpASK revAmp/Get V111pFastBacHTNdeI-His8-MBP-TEV-Sali,NotI-stopSalI-NotIHis8-MBP-TEVpASk forpASK revAmp/Get V112pFastBacHTNdeI-His8-MBP-TEV-Sali,NotI-XhoI-His6-stopSalI-NotIHis8-MBP-TEVpASk forpASK revAmp/Get V113pColdIINdeI-His8-MBP-TEV-SalI,NotI-stopSalI-NotIHis8-MBP-TEVpCold forpCold revAmp V114pColdIINdeI-MBP-TEV-SalI,NotI-stopSalI-NotIMBP-TEVpCold forpCold revAmp V115pColdIINdeI-XhoI-His6-stopunknownnonepCold forpCold revAmp V116pET30aNdeI-B562-XhoI-His6-stopunknownB562T7 PromoterT7 TerminatorKan V117pCold V118pGADzaA V119FBQHHis8-TEV-BamHI-XhoIBamHI-XhoIHis8-TEVFBQ-FFBQ-RAmp V120FBQHGST-TEV-BamHI-Xho IBamHI-XhoIGST-TEVFBQ-FFBQ-RAmp V121FBQHHis-MBP-TEV-BamHI-XhoIBamHI-XhoIHis-MBP-TEVFBQ-FFBQ-RAmp V122PET30aNdeI- His8- GB1 -Sal I -Not I-stopSal I-Not IHis8- GB1T7-PT7-TKanXY V123pCDNA3.1CMV-T7-FLAG-MCS-StopXhoI-BamH I-EcoR I-Not IFLAGT7/CMVBGH-RAmpXY V124pCDNA3.1CMV-T7-HA-MCS-StopXhoI-BamH I-EcoR I-Not IHAT7/CMVBGH-RAmpXY V125pCDNA3.1CMV-T7-Strep-MCS-StopXhoI-BamH I-EcoR I-Not IStrepT7/CMVBGH-RAmpXY V126pCDNA3.1CMV-T7-SP-3XFLAG-MCS-StopXhoI-BamH I-EcoR I-Not I3XFLAGT7/CMVBGH-RAmpXYSP=signal peptideV127pCDNA3.1CMV-T7-SP-FLAG-Clover-MCS-StopXhoI-BamH I-EcoR I-Not IClover/FLAGT7/CMVBGH-RAmpXYSP=signal peptideV128pCDNA3.1CMV-T7-SP-HA-MCS-StopXhoI-BamH I-EcoR I-Not IHAT7/CMVBGH-RAmpXYSP=signal peptideV129pCDNA3.1CMV-T7-SP-Strep-mRubby2-MCS-StopXhoI-BamH I-EcoR I-Not IStrep/mRubby2T7/CMVBGH-RAmpXYSP=signal peptideV130pCDNA3.1CMV-T7-SP-MCS-StopXhoI-BamH I-EcoR I-Not IStrepT7/CMVBGH-RAmpXY V131pCDNA3.1CMV-T7-Strep-mRubby2-MCS-StopXhoI-BamH I-EcoR I-Not IStrep/mRubby2T7/CMVBGH-RAmpXY V132pCDNA3.1CMV-T7-FLAG-Clover-MCS-StopXhoI-BamH I-EcoR I-Not IClover/FLAGT7/CMVBGH-RAmpXY V133pCDNA3.1CMV-T7-Strep-NEGFP-MCS-StopXhoI-Not IStrep/NEGFPT7/CMVBGH-RAmpXY V134pCDNA3.1CMV-T7-MCS-CEGFP-Myc-StopXhoI-Not IMyc/CEGFPT7/CMVBGH-RAmpXY V135PRSFT7-His6-MCS-Stop/T7-MBP-TEV-MCS-His6-StopBamH I-Not I/Hind III-EcoR IHis/MBPZ21Z20KanXY V136plsecSUMOstarGP67A-HIs6-SUMOstar-SalI,NotI-stopSal I-Not IHisY935FXJ57Amp V137plsecSUMOstarGP67A-HIs6-SUMOstar-MBP-TEV-SalI,NotI-stopSal I-Not IHis/MBPZ8FXJ57Amp V138plsecSUMOstarGP67A-HIs6-TEV-SalI,NotI-stopSal I-Not IHis-TEVZ8FXJ57Amp V139plsecSUMOstarGP67A-HIs6-SalI,NotI-stopSal I-Not IHisFXJ66 Amp V140PTT5IFNA1-SalI,XbaI-His6-stopSal I-Xba IHisPTT5-FPTT5-RAmp V141PTT5IFNA1-SalI,XbaI-Fc-stopSal I-Xba IFcPTT5-FPTT5-RAmp V142PTT5IFNA1-SalI,XbaI-Fc-TEV-stopSal I-Xba IFc-TEVPTT5-FPTT5-RAmp V143plsecSUMOstarGP67A-HIs6-SUMOstar-EGFP-TEV-SalI,NotI-stopSal I-Not IHis-EGFP-TEVZ8FXJ57Amp 1. DH5α菌株2. TOP10菌株3. HB101菌株4. XL1-Blue菌株5. DH10B菌株6. B834菌株7. BL21(DE3)菌株8. BL21(DE3) pLysS菌株9. BL21(DE3) RosettaTM菌株（Novagen)10. BL21 Codon plusTM(DE3)-RIPL (Stratagene)11. JM109菌株12. 293T细胞 Cell StrainsResistanceUsage1Sf9none包装病毒2Hi5none昆虫细胞表达蛋白3HEK293none表达包装病毒4293Tnone工具细胞5L929none分泌gmcsf，促进巨噬细胞分化 ▲ 表格中”No found” 部分因未查到其细胞抗性，建议实验中视为无抗性处理，其具体特性请自行查询。 1. MBP-phusion Protein Crystallization Screen2. Wizard™ I/Ⅱrandom sparse matrix crystallization screen3. Wizard™ III/Ⅳrandom sparse matrix crystallization screen4. The Anions Suite5. The Nucleix Suite6. The PACT Suite7. The Protein Complex Suite8. Natrix HT（HR2-131）9. SaltRx HT（HR2-136）10. PEGRx HT（HR2-086）11. Crystal Screen HT（HR2-130）
